Part:BBa_K1371004
AT-ACP-L1(loading module)
The leading role of the loading part is Acyltransfer-ase, AT domain. In this part, we will introduce the function of AT domain by analyzing the chemical reactions between AT domain, ACP domain and polyketide chains.
The function of AT domain is activating the extended units, propionyl groups which will be combined with the polyketide chains.
In Loading, Proplonyl-CoA reacts with the oxhydryl on the pecific binding sites of AT domains and binds to AT domain, and then CoA-SHs are displaced. At this time, proplonyl groups have been activated. They are transferred onto the ACP domains in Loading and anchored there.
In Module 1 or Module 2, AT domains anchor the proplonyl groups of (2S)Methylmalonyl-CoA to the ACP domains in Module 1 or Module 2 by the same mechanism. The proplonyl groups anchored wait for the catalysis of KS domain in the next step.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 507
Illegal AgeI site found at 318 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1432
| n/a | AT-ACP-L1(loading module) |